Neven A. Ebrahim
Assistant Professor at Taibah University, Kingdom of Saudi ArabiaPresentation Title:
The effect of Sofosbuvir on rats’ ovaries and the possible protective role of vitamin E
Abstract
Hepatitis C virus (HCV) infection is a major health problem worldwide and its eradication is mandatory. Direct acting HCV polymerase inhibitors, such as Sofosbuvir (SOF), is an effective regimen. However, it has some side effects like mutagenesis, carcinogenesis, the impairment of testicular function and fertility. It is important to evaluate the safety of SOF on the ovaries as well, as there are no studies yet. Increasing the production of Reactive Oxygen Species (ROS), causes oxidative stress, which affects ovulation process, female reproduction, and fertility. Accumulation of SOF in the cells was demonstrated to promote ROS generation. Vitamin E (Vit E) is an antioxidant agent that has an essential role in the female reproductive system, its deficiency can cause infertility. We explored the effect of SOF treatment alone and co-treated with Vit E on ovarian ROS level and ovarian morphology experimentally using twenty-four virgin female Sprague-Dawley rats, average age 6–8 weeks (each 150–250 g weight), through biochemical and immunohistochemical studies. Significant changes in oxidative stress markers; nitric oxide and malondialdehyde lipid peroxidation, antioxidant enzymes; catalase, super oxide dismutase, and reduced glutathione, proliferating markers; proliferation cell nuclear antigen and Ki-67 antigen and caspase 3 apoptotic marker were demonstrated. It was shown that SOF induced oxidative stress, as a persuader of ovarian cytotoxicity with its subsequent ovarian dysfunctions. The essential role of Vit E as an antioxidant agent in protecting the ovarian tissue from the effect of oxidative stress markers and preserving its reproductive functions was also displayed. This could be guidance to add Vit E supplements to SOF regimens to limit its injurious effect on the ovarian function.
Biography
I am an assistant professor of Human Anatomy and Embryology with Taibah Medical School KSA. I also worked as a postdoctoral research fellow with Harvard Medical School, USA till 2019. I am also a lecturer of Human Anatomy with the Faculty of Medicine at Mansoura University, Egypt, from which I graduated in 2004. I have obtained a PhD in Human Anatomy and cell biology from the University of Nottingham, UK in 2016, where I also worked as a senior demonstrator then a lecturer of Human Anatomy. My research focused on stem cell, tissue engineering and vascular repair using a variety of techniques, including immune-fluorescent, flow cytometry, western blotting, ELISA, confocal, real-time microscopy and Electron microscopy.
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