Mehdi Mehdizadeh
Iran University of Medical Sciences, IranPresentation Title:
Changes in the transcriptomic profile of cumulus cells under the influence of cumulus-oocytes complex preincubation
Abstract
Pre-incubation of the
Cumulus-Oocyte Complex (COCs) may lead to better function of Cumulus Cells
(CCs) and higher oocyte quality by changing the transcriptomic profile of CCs.
140 cumulus cell samples were isolated from 12 participants and divided into two
groups based on pre-incubation time. In the T0 group, the COCs were immediately
dissected to separate the CCs from around the oocytes. In the T2 group, CCs
were prepared after 2h of incubation. Then, the transcriptomic profile of the
CCs of the non-pre-incubation group was compared to the 2h pre-incubation
group. Confirmation of RNA sequencing results was done via qRT PCR. The CCs
transcriptome analysis showed 17 genes were downregulated and 22 genes
upregulated in the T2 group compared to the T0 group. Also, the pathways
related to ATP production (oxidative phosphorylation, electron transport chain,
and Mitochondrial complex I assembly model OXPHOS system), TNF-alpha signaling
pathway, and glucocorticoid receptor pathway increased in the T2 group compared
to the T0 group. Also, the TGF-β pathway was decreased in the T2 group compared
to the T0 group. This study showed that 2 h pre-incubation leads to changes in
important pathways in CCs, which positively affects oocyte quality.
Biography
Mehdi Mehdizadeh is an esteemed
academic in the field of anatomical sciences, with a notable background in
medical education and research. He completed his PhD in anatomical sciences at
Tehran University of Medical Sciences (TUMS), where he also obtained his M.Sc.,
demonstrating outstanding academic performance. He further honed his expertise
through a fellowship in transgenic animals at the German Research Center for
Biotechnology in 2004 and a sabbatical at the University of Manitoba, Canada,
in 2012. Since 1995, he has been a dedicated educator at the Iran University of
Medical Sciences, teaching gross anatomy, embryology, genetics, and molecular
biology to a diverse range of students, including undergraduates and
postgraduates in medical, rehabilitation, and life sciences. His contributions
to the academic community are marked by his commitment to advancing knowledge
in anatomical and biological sciences through both teaching and research
excellence.
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