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Mehdi Mehdizadeh

Iran University of Medical Sciences, Iran

Presentation Title:

Changes in the transcriptomic profile of cumulus cells under the influence of cumulus-oocytes complex preincubation

Abstract

Pre-incubation of the Cumulus-Oocyte Complex (COCs) may lead to better function of Cumulus Cells (CCs) and higher oocyte quality by changing the transcriptomic profile of CCs. 140 cumulus cell samples were isolated from 12 participants and divided into two groups based on pre-incubation time. In the T0 group, the COCs were immediately dissected to separate the CCs from around the oocytes. In the T2 group, CCs were prepared after 2h of incubation. Then, the transcriptomic profile of the CCs of the non-pre-incubation group was compared to the 2h pre-incubation group. Confirmation of RNA sequencing results was done via qRT PCR. The CCs transcriptome analysis showed 17 genes were downregulated and 22 genes upregulated in the T2 group compared to the T0 group. Also, the pathways related to ATP production (oxidative phosphorylation, electron transport chain, and Mitochondrial complex I assembly model OXPHOS system), TNF-alpha signaling pathway, and glucocorticoid receptor pathway increased in the T2 group compared to the T0 group. Also, the TGF-β pathway was decreased in the T2 group compared to the T0 group. This study showed that 2 h pre-incubation leads to changes in important pathways in CCs, which positively affects oocyte quality.

Biography

Mehdi Mehdizadeh is an esteemed academic in the field of anatomical sciences, with a notable background in medical education and research. He completed his PhD in anatomical sciences at Tehran University of Medical Sciences (TUMS), where he also obtained his M.Sc., demonstrating outstanding academic performance. He further honed his expertise through a fellowship in transgenic animals at the German Research Center for Biotechnology in 2004 and a sabbatical at the University of Manitoba, Canada, in 2012. Since 1995, he has been a dedicated educator at the Iran University of Medical Sciences, teaching gross anatomy, embryology, genetics, and molecular biology to a diverse range of students, including undergraduates and postgraduates in medical, rehabilitation, and life sciences. His contributions to the academic community are marked by his commitment to advancing knowledge in anatomical and biological sciences through both teaching and research excellence.